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KMID : 0525719990040010050
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Journal of Chitin and Chitosan
1999 Volume.4 No. 1 p.50 ~ p.56
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Properties of chitinases from gizzards of broiler ( Gallus gallus L )
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한범구/Han BK
백제현/조성준/박인호/이우진/조도현/Baek JH/Cho SJ/Park IH/Lee WJ/Jo DH
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Abstract
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Purification of two chitinases, LMC(low molecular weight chitinase) and HMC(high molecular weight chitinase)from gizzards of broiler was carried out using ion-exchange chromatography Eel-filtration chromatofocusing and hydrophobic interaction chromatography. They were purified about 180 folds with a 3~5% yield. The molecular weight of LMC and HMC were 48.2 kDa and 57.8 kDa, respectively. Both were endotype chitinases having optimum pH of 4~5 and optimum temperature of 60℃. Both enzymes had different Km and Vmax values toward [3H]- caitin. The NH2 -terminal sequences of LMC and HMC shared high level of homology with those reported in animal chitinases.
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KEYWORD
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